DeadEnd™ Fluorometric TUNEL System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- When there was a little or poor staining, optimise permeabilization step by adjusting incubation time with permeabilization agent. - When there is a loss of tissue from slides, decrease Proteinase K incubation time.

Downstream tips
- When there was a little or poor staining, optimise permeabilization step by adjusting incubation time with permeabilization agent. - When there is a loss of tissue from slides, decrease Proteinase K incubation time.

Publication protocol

Clinical-grade gefitinib was kindly provided by AstraZeneca. Cisplatin was purchased from Sigma-Aldrich. The anti-phospho-Akt, anti-Akt, anti-phospho ERK, anti-ERK, anti-phospho EGFR, anti-EGFR, anti-HER2, and anti-PARP antibodies were obtained from Cell Signaling. The anti-HER3 antibody was purchased from Upstate Cell Signaling Solutions. The terminal deoxynucleotidyltransferase-mediated dUTP nick end-labeling (TUNEL) kit (DeadEndTM Fluorometric TUNEL System®) was obtained from Promega.

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer using DeadEnd™ Fluorometric TUNEL System from Promega.

Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Fluorometric TUNEL System below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer using DeadEnd™ Fluorometric TUNEL System from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.