Anti-Villin antibody [3E5G11] - N-terminal (ab201989)

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Villin, Pro-Ex-C, ER and PR expressions were assessed using immunohistochemistry in sections from all the 45 blocks of paraffin. We analyzed correlations between the levels of Villin, Pro-Ex-C, ER and PR expressions and ability of panel of both markers in differentiation between ECA and EMC; we also analyzed the predictive role of marker expression. The streptavidine-biotin technique was used for immunohistochemical staining19. We incubated sections with primary; rabbit monoclonal anti- Pro-Ex-C-antibody (MCM2 26H6.19), mouse monoclonal anti-Villin-antibody (clone 1D2C3, ab739 , dilution 1:100) at 4°C overnight (Abcam, Cambridge, MA, USA), antiestrogen receptor (clone GF11, dilution 1:50, Novocastra Laboratories, Newcastle upon Tyne, United Kingdom) and anti-progesterone receptor (clone 16, dilution 1:200, Novocastra), followed by incubation with secondary antibodies. Sections from small intestine were used as a positive control for villin, and sections from EMC were used as the positive control for ER and PR receptor expression20,21. For negative controls, the primary antibodies were removed but replaced with phosphate-buffered saline. We evaluated the stained slides without previous identification of the clinical and pathological parameters.

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