Glucocorticoid Receptor (D8H2) XP® Rabbit mAb #3660

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Human OvCa tissue microarrays were constructed by the Human Tissue Research Center (HTRC) of the University of Chicago with Institutional Review Board Approval as previously described (16, 17). All samples underwent pathology review to establish ovarian tumor subtype. H&E staining to confirm tumor as well as percentage and intensity of nuclear GR, ER, PR, and AR staining was performed on the formalin-fixed paraffin-embedded specimens as above. The following antibodies were used anti-GR-XP antibody (D8H2, 1:500, Cell Signaling), anti-ER-alpha antibody (6F11, 1:40, DAKO) anti-PR antibody (16, 1:200, DAKO) and anti-AR antibody (AR441, 1:300, DAKO). A modification of the H- (Histological) score as previously published (18, 19) was calculated to quantify GR, ER, PR, and AR expression in the primary human HGS-OvCa tissue samples. The H-score was calculated by multiplying the intensity of nuclear staining (0: no staining, 1: weak, 2: moderate and 3: strong staining) by the percentage of the tumor cells staining. The minimum H-score was 0 and the maximum was 300.

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