ApopTag Fluorescein in Situ Apoptosis Detection Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Fix 5 x 10^7 cells/mL cells in 1% PFA in PBS, pH 7.4 - After applying Apply Equilibration Buffer and Working Strength TdT Enzyme , incubate at 37C for 1 hour.

Protocol tips
- Fix 5 x 10^7 cells/mL cells in 1% PFA in PBS, pH 7.4 - After applying Apply Equilibration Buffer and Working Strength TdT Enzyme , incubate at 37C for 1 hour.

Publication protocol

Tunel staining was performed with the use of an ApopTag Fluorescein Direct in Situ Apoptosis Detection kit (Chemicon, UK) according to the manufacturer’s instructions. Images were visualized by specific green fluorescence and nuclei by 4'-6-diamidino-2 phenylindole (DAPI) (nuclear counterstain). The TUNEL positives cells were examined with a microscope (Nikon TE-2000 U) and images were acquired with a digital camera (Nikon).

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Manufacturer protocol

Download the product protocol from Millipore for ApopTag Fluorescein in Situ Apoptosis Detection Kit below.

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