MLH1 antibody [G168-15]

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

Tumor specimens were fixed in 10% neutral-buffered formalin for 48 h, embedded in paraffin, and cut into 4-μm-thick sections, and then mounted on silane-coated glass slides. Antigen-retrieval was done by autoclaving (121 °C) for 5 min in pH 9 Antigen Retrieval Liquid (Nichirei, Tokyo, Japan) for MUC2, MUC5AC, and hMSH2, and by microwave irradiation for 10 min in pH 9 Antigen Retrieval Liquid for human mutL homolog 1 (hMLH1). Primary antibodies used were the mouse monoclonal antibody for MUC2 (1:100 dilution; clone Ccp58, Novocastra, Newcastle Upon Tyne, United Kingdom), the mouse monoclonal antibody for MUC5AC (1:100 dilution; CLH2, Novocastra), the rabbit anti-MLH1 monoclonal antibody (1:400 dilution; EPR3894, GeneTex, San Antonio, TX, United States), and the rabbit anti-MSH2 polyclonal antibody (1:200 dilution; 15520-1-AP, Proteintec, Chicago, IL, United States). Samples were treated overnight with each primary antibody at 4 °C. Immunostaining was performed blindly by an investigator (Fukuda K) who was unaware of the clinical information using an N-Histofine Simple Stain MAX-PO kit (Nichirei).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Immunohistochemistry Human - MLH1 using MLH1 antibody [G168-15] from GeneTex.

Manufacturer protocol

Download the product protocol from GeneTex for MLH1 antibody [G168-15] below.

Videos

Check out videos that might be relevant for performing Immunohistochemistry Human - MLH1 using MLH1 antibody [G168-15] from GeneTex. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.