1ml NCL-L-Ki67-MM1

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Publication protocol

IHC was performed on sequential tissue sections according to standard protocol as previously described (Valentijn et al., 2013). Primary antibodies (mouse pan-monoclonal anti-cytokeratin (C2562, Sigma-Aldrich, Dorset, UK) at 1:4000, goat polyclonal anti-SOX9 (af3075, R&D Systems, Abingdon,UK) at 1:400, mouse monoclonal anti-Ki67 (NCL-Ki67-MM1, Novocastra, Newcastle, UK) at 1:200, mouse monoclonal anti-SSEA-1 (125601/2, Biolegend, San Diego, CA) at 1:800 dilution) were incubated overnight at 4°C in a humidified chamber. All slides were scanned using an Aperio CS2 scanner (http://www.leicabiosystems.com/digital-pathology/aperio-digital-pathology-slide-scanners/products/aperio-cs2/) and analysed using spectrum, ScanScope®.

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