7ml p53 Bond RTU Primary

Immunohistochemistry Human - p53

Experiment

Immunohistochemistry Human - p53

Product

7ml p53 Bond RTU Primary from Leica

Manufacturer

Leica

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Publication protocol

Analysis of TP53 gene status can be made by direct observation of p53 protein in tissues by immunohistochemical techniques. IHC staining was performed with specific monoclonal antibodies anti-p53 (CM1, Novocastra, Newcastle, UK). We evaluated immune reactivity to p53 depending on the percentage of positive tumor cells. It were taken into account only the tumor cells with nuclear stain, cytoplasmic stain was not considered.

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