Anti-Notch1 antibody (ab27526)

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Immunohistochemistry (IHC) and immunofluorescence assays were performed as previously described [21]. IHC scores were assessed using a semiquantitative grading system [22]. The appropriate antibodies against Notch1 (Abcam), E-cadherin (CST), N-cadherin (CST), Fibronectin (Abcam) and Vimentin (Abcam) were used. Immunofluorescence assays were visualized using Goat anti-Rabbit Alexa Fluor® 594-conjugated (ZSGB-BIO) or Goat anti-Mouse Fluorescein–conjugated (ZSGB-BIO) antibodies. Cell nuclei were counterstained using Hoechst 33258 (Thermo Fisher Scientific). Representative images were captured, and they were analysed by Olympus FV1000 Digital laser scanning microscopy.

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