HES1 Mouse Monoclonal Antibody [Clone ID: OTI1B5]

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

From tissue obtained before chemotherapy, 1 representative block of formalin-fixed, paraffin-embedded tissue was selected for immunohistochemistry (IHC). Following review of published literature, 9 antibodies were selected to detect Survivin, PLK-1, Cytokeratin19 (CK19), N-Myc, Yap, Notch2, Hes1, Hes5, and c-Myc. IHC was performed manually (Survivin, CK19, Yap, Notch2, Hes1, and Hes5) or automated (N-Myc, C-Myc, and PLK-1) following antigen retrieval in citrate buffer at pH 6.0. The various antibodies used are detailed in Table 1.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Immunohistochemistry Human - Hes1 using HES1 Mouse Monoclonal Antibody [Clone ID: OTI1B5] from OriGene.

Manufacturer protocol

Download the product protocol from OriGene for HES1 Mouse Monoclonal Antibody [Clone ID: OTI1B5] below.

Videos

Check out videos that might be relevant for performing Immunohistochemistry Human - Hes1 using HES1 Mouse Monoclonal Antibody [Clone ID: OTI1B5] from OriGene. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.