CC10 Antibody (E-11): sc-365992

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For immunofluorescent (IF) staining, sections were incubated with primary polyclonal antibodies of P63 (1:50, anti-mouse, ab735, Abcam, United States), CC10 (1:500, anti-mouse, sc-365992, Santa Cruz Biotechnology, United States) and SPC (1:100, anti-mouse, sc-518029, Santa Cruz Biotechnology, United States), respectively. We performed triple co-staining with TTF-1 (1:300, anti-rabbit, ab76013, Abcam, United States), α-tubulin (1:100, anti-chicken, ab89984, Abcam, United States) and one of the above-mentioned markers for overnight incubation at 4°C, followed by incubation with Alexa Fluor 488-, Alexa Fluor 555-, and Alexa Fluor 647-conjugated secondary antibodies (1:500, Life Technologies, Carlsbad, CA, United States) at 37°C for 1 h. The nuclei were visualized by staining with 4′-6-diamidino-2-phenylindole (Life Technologies, Carlsbad, CA, United States). Images were acquired with fluorescence microscopy (Leica DM6, Wetzlar, Germany).

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