[KO Validated] SOX2 Rabbit pAb (A0561)

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Publication protocol

For immunohistochemical (IHC) analysis, human glioma tissues and normal brain tissues were examined (Table ​(Table1).1). These experiments were approved by Human Ethics Committee of Hunan Normal University and informed consent was obtained from all patients. The IHC analysis was performed on polyformalin-fixed and paraffin-embedded tissues as previously described 22, 28. Tissue sections were incubated with indicated primary antibodies against AP-2α (3B5) (A0416) (1:200), Nanog (AF5388) (1:200), Sox2 (A0561) (1:200), CD133 (A0219) (1:200), p-STAT3 (AF3293) (1:100, AbClonal Technology) and Ki67 (9449) (1:200, cell signaling technologies) or normal mouse IgG control (sc-2025) (1:200, Santa Cruz, CA). The percentage of tumor cells stained was scored as 0 (no cell staining), 1 (≤30%), 2 (31-60%) and 3 (61-100%). Staining between two score values was given 0.5.

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