Monoclonal Mouse Anti-Human E-Cadherin (Dako Omnis) Clone NCH-38

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

"IHC was used for assessing the expression of E-cadherin. One 4-µm-thick section was cut from 1 representative paraffin block of each case. The sections
were floated onto salinized glass slides and dried out at 37°C overnight, and then kept in 60°C for 1 hr, before de-paraffinization in xylene and rehydration through
graded ethanol. All sections were subjected to microwave heating at 850 W for 22 min in pH 6.0 citrate buffer and cooled in running water. Antibody used was E-cadherin clone CL NCH-38 (Dako, Poland), dilution 1:50 at room temperature. IHC staining was carried out using a HRP polymer detection envision method (Dako,
EnVision+System, Poland). Diaminobenzidine (DAB) was used as chromogen and sections were counterstained with Harris’ hematoxylin. Appropriate positive and negative controls omitting the primary antibodies were included with each slide run. "

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Immunohistochemistry Human - E-Cadherin using Monoclonal Mouse Anti-Human E-Cadherin (Dako Omnis) Clone NCH-38 from Agilent Technologies.

Manufacturer protocol

Download the product protocol from Agilent Technologies for Monoclonal Mouse Anti-Human E-Cadherin (Dako Omnis) Clone NCH-38 below.

Videos

Check out videos that might be relevant for performing Immunohistochemistry Human - E-Cadherin using Monoclonal Mouse Anti-Human E-Cadherin (Dako Omnis) Clone NCH-38 from Agilent Technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.