Annexin V-FITC Apoptosis Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Always stain cells with Annexin before fixation (makes cell membrane leaky)

Protocol tips
- Always stain cells with Annexin before fixation (makes cell membrane leaky)

Publication protocol

Apoptosis of the cryptotanshinone or tanshinone IIA-treated cells was quantitated by double staining with Annexin V-FITC and PI using the Annexin V-Apoptosis Detection kit (Biovision, Milpitas, CA, USA) according to the manufacturer's instructions. Apoptotic cells were analyzed by FACSCalibur (Becton Dickinson, San Jose, CA, USA) to be defined as those positive for Annexin V with or without PI staining.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Apoptosis assay cell type - K562 using Annexin V-FITC Apoptosis Kit from Biovision.

Manufacturer protocol

Download the product protocol from Biovision for Annexin V-FITC Apoptosis Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Apoptosis assay cell type - K562 using Annexin V-FITC Apoptosis Kit from Biovision. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.