Annexin V-FITC Apoptosis Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cells were pretreated with CM and CF at concentrations required for 50% inhibition of growth	- Always stain cells with Annexin before fixation (makes cell membrane leaky)

Upstream tips
- Cells were pretreated with CM and CF at concentrations required for 50% inhibition of growth
Protocol tips
- Always stain cells with Annexin before fixation (makes cell membrane leaky)

Publication protocol

To detect the apoptosis induction in HT-29 cells, for each assay, the cells were treated with CM and CF at concentrations required for 50% inhibition of growth of HT-29 cells (IC50) for 24 h (40 µg/mL). Untreated cells with 0.05% DMSO (v/v) vehicle served as negative control.

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Manufacturer protocol

Download the product protocol from Biovision for Annexin V-FITC Apoptosis Kit below.

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