FITC Annexin V Apoptosis Detection Kit I

Protocol tips

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	- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Publication protocol

Apoptosis and necrosis of HT-29 cells were assessed with an Annexin V-FITC Apoptosis Detection Kit (BD Company, USA). The cells were stained with annexin V-fluorescein isothiocyanate (Annexin V-FITC) and propidium iodide (PI) for analyses by flow cytometry. The FITC and PI fluorescence were measured through 530 nm and 585 nm emission, respectively. Positioning of quadrants on Annexin V/PI dot plots was performed. The living cells (Annexin V−/PI−), early apoptotic cells (Annexin V+/PI−), late apoptotic (Annexin V+/PI+), and necrotic cells (Annexin V−/PI+) were distinguished. Therefore, the total apoptotic proportion included the percentage of cells with fluorescence Annexin V+/PI− and Annexin V+/PI+ (Lee et al. 2008 Lee, DH, Szczepanski, M and Lee, YJ. 2008. Role of Bax in quercetin-induced apoptosis in human prostate cancer cells. Biochem Pharmacol, 75: 2345–2355.



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