DMEM/F-12

Protocol tips

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	Seed cancer cells at a density of 1000cells/ml Use of ultra-low adhesion plates Count mammospheres with diameter > 75μm

Protocol tips
Seed cancer cells at a density of 1000cells/ml Use of ultra-low adhesion plates Count mammospheres with diameter > 75μm

Publication protocol

Cancer cells (1000 cells/ml) were cultured in ultra-low adhesion plates (Corning) in serum-free DMEM-F12 (GIBCO), containing B27 (1:50, Invitrogen), 20 ng/mL EGF (BD Biosciences), 0.4% bovine serum albumin (Sigma), and 4 mg/ml insulin (Sigma). After culturing for 10 days, mammospheres with diameter > 75μm were counted. Six replicate wells were included in each analysis and at least three independent experiments were conducted.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for DMEM/F-12 below.

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