DMEM/F-12, no phenol red

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Seed 1x10 3 cells/well

Protocol tips
Seed 1x10 3 cells/well

Publication protocol

1x103 cells were counted and resuspended in 2 mL of mammosphere media (50 mL of Phenol-free DMEM (GIBCO, cat# 21041-025) supplemented with 1% L-glutamine, 1% penicillin/streptomycin, 1X B27 supplement minus Vitamin A (GIBCO, cat. #12587-010), 20 ng/ml rhEGF (R&D Systems, cat #236-EG-200) and 20 ng/ml bFGF (Invitrogen, Carlsbad, CA)) and cultured in a 6-well plate with an ultra-low attachment surface (Costar). The plate was left undisturbed for ten days in a 37°C incubator. Mammospheres were counted and measured using an inverted Axiovert 40 CFL microscope at 20X magnification.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing 3D Cell Culture Media BT-549 cells-Mammospheres using DMEM/F-12, no phenol red from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for DMEM/F-12, no phenol red below.

Videos

Check out videos that might be relevant for performing 3D Cell Culture Media BT-549 cells-Mammospheres using DMEM/F-12, no phenol red from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.