Annexin-V-FLUOS Staining Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1×10^6 cells were seeded and infected	- Cells were double stained with Annexin-V-FITC and propidium iodide (PI) at different time points after infection

Upstream tips
- 1×10^6 cells were seeded and infected
Protocol tips
- Cells were double stained with Annexin-V-FITC and propidium iodide (PI) at different time points after infection

Publication protocol

Approximately 1×106 NMII infected and unpinfected THP-1 cells were double stained with Annexin-V-FITC and propidium iodide (PI) at different time points post infection by using the Annexin-V-FLUOS Staining Kit according to the manufacturer's protocol (Roche). Following staining, the cells were washed twice with FACS buffer (0.5% BSA) and fixed with 1% paraformaldehyde for 15 min. Fluorescence was detected using a fluorescence-activated cell sorter (FACS Calibur, Becton-Dickinson, Palo Alto, CA) to analyze necrotic (PI+), non-apoptotic (negative for both dyes), early apoptotic (Annexin+/PI−) and late apoptotic cells (Annexin+/PI+). Fluorescence parameters were gated using unstained and single-stained uninfected control cells and 10,000 cells were counted for each sample.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Apoptosis assay cell type - THP-1 using Annexin-V-FLUOS Staining Kit from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Annexin-V-FLUOS Staining Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Apoptosis assay cell type - THP-1 using Annexin-V-FLUOS Staining Kit from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.