HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid

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Publication protocol

Cell culture: Authenticated human glioma cell lines U251 and U87 were cultured in Dulbecco's modified Eagle's medium and F‐12 1 : 1 (DME‐F12; Hyclone, Thermo Fisher Scientific, Burlington, ON, Canada) plus 10% fetal bovine serum (FBS; Sigma, Oakville, ON, Canada). Patient GB cells were isolated from tissues obtained from GB patients treated at the Health Science Centre (Glogowska et al., 2013). The study was approved by the University of Manitoba and Pathology ethics boards, and written consent was obtained from the patients. Tumor sphere formation was performed and the mouse GB‐initiating cell line NF53 was established as described (Friedmann‐Morvinski et al., 2012; Thanasupawat et al., 2015).

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