DMEM with Glucose and L-Glutamine

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Cell culture and reagents: A glioma cell line, U87MG, was purchased from the American Type Culture Collection (ATCC, USA), and a human promyeloblast, HL-60, was gifted from Dr. Taehoon Chun (Korea University, Korea). All established glioma cell lines were maintained in Dulbecco’s modified Eagle’s medium (Lonza, Switzerland) supplemented with 10% fetal bovine serum (Biotechnics Research Inc., USA), 1% penicillinstreptomycin (Lonza), 1% l-glutamine (Lonza), and gentamicin sulfate (50 μg/ml; Mediatech, USA). HL-60 was maintained in Roswell Park Memorial Institute (RPMI)-1640 medium (Lonza) supplemented with 5% fetal bovine serum, 1% penicillin-streptomycin, and 14 mL of 1M HEPES (Hy-Clone™; GE Healthcare Sciences, USA). After serum starvation, the cells were treated with 10 ng/mL recombinant human IL-1β (BD Biosciences, USA) at different time intervals (12, 4, and 1 h). Cells were harvested at the same time and analyzed. Limiting dilution assay (LDA): For LDA, the indicated number of cells per well was seeded in 96-well plates, and each well was examined for the formation of tumor spheres.

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