RosetteSep™ Human Monocyte Enrichment Cocktail

Protocol tips

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		Enriched cells were labeled with CD3, CD19, CD20, CD56, CD66b, HLA-DR, CD14, and CD16 antibodies before sorting classical monocytes by FACS AriaII (BD).

Downstream tips
Enriched cells were labeled with CD3, CD19, CD20, CD56, CD66b, HLA-DR, CD14, and CD16 antibodies before sorting classical monocytes by FACS AriaII (BD).

Publication protocol

Blood was collected from healthy volunteers and mononuclear phagocytes enriched using RosetteSep Human Monocyte Enrichment Cocktail (STEMCELL Technologies) following the manufacturer’s instructions. Enriched cells were labeled with CD3, CD19, CD20, CD56, CD66b, HLA-DR, CD14, and CD16 antibodies before sorting classical monocytes by FACS AriaII (BD). Sorted classical monocytes were adoptively transferred intravenously into MISTRG mice (Rongvaux et al., 2014; Deng et al., 2015). Peripheral blood was collected by cardiac puncture under terminal anesthesia; erythrocytes were lysed by ACK (Lonza). The leukocyte fraction was stained and analyzed by flow cytometry. The fate of the classical monocytes were analyzed at selected time points after transfer identified as human mCD45−hCD45+HLA-DR+CD33+Lin− by flow cytometry.

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Manufacturer protocol

Download the product protocol from STEMCELL technologies for RosetteSep™ Human Monocyte Enrichment Cocktail below.

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