Dead Cell Apoptosis Kit with Annexin V FITC and PI

Apoptosis assay cell type - OECM-1 human oral squamous cell carcinoma

Experiment
Apoptosis assay cell type - OECM-1 human oral squamous cell carcinoma
Product
Dead Cell Apoptosis Kit with Annexin V FITC and PI from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- 2 × 10^4 cells/well were seededfor assay.

- cells were treated with LPLI
Protocol tips
- Cells are stained with PI and annexin V-FITC and incubated on ice for 10–15 min

Publication protocol

For the apoptosis assay, the detached oral cancer cells treated with LPLI were washed with PBS and stained with propidium iodide (PI) and annexin V-FITC (Invitrogen, V13241) on ice for 10–15 min. The stained cells were analyzed using a FACScan flow cytometer (Becton Dickinson) and the number of apoptotic cells was quantified using FlowJo software (Tree Star). Alternatively, cells were seeded into 96-well white plate (2 × 104 cells/well) for overnight and exposed to LPLI in the presence or absence of autophagy inhibitors. The cells were then recovered for 24 h and lyzed with Caspase-Glo 3/7 (Promega, G8091) to measure luminescent signal for 1 h. The net relative luminescence units (RLU) between time 0 and 1h were used to reflect caspase 3/7 activity in treated cells.

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Papers

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Paper title
RelA-Mediated BECN1 Expression Is Required for Reactive Oxygen Species-Induced Autophagy in Oral Cancer Cells Exposed to Low-Power Laser Irradiation
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Dead Cell Apoptosis Kit with Annexin V FITC and PI below.

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