MojoSort™ Human Pan Monocyte Isolation Kit

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		Macrophages were obtained from human peripheral blood monocytes. To obtain macrophages, monocytes were seeded on culture plastic and incubated for 7 days in DMEM/F12 medium with 2% fetal bovine serum.

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Macrophages were obtained from human peripheral blood monocytes. To obtain macrophages, monocytes were seeded on culture plastic and incubated for 7 days in DMEM/F12 medium with 2% fetal bovine serum.

Publication protocol

The cells of human acute myeloid leukemia THP‑1, obtained from the all-Russian collection of cell cultures (Institute of Cytology of the RAS, St. Petersburg), were used as an object of this study. The THP‑1 cells (hereinafter THP-1wt) were cultured in RPMI 1640/F12 medium (Sigma, United States) with 20% fetal bovine serum (Gibco, United States), 80 μg/mL gentamicin sulfate (Sigma, United States), 20 μg/mL diflucan (Pfizer, United States) at 37°C in the air with 5% CO2. Monocyte-like cells were obtained by treating the cells with retinoic acid (ATRA). For this purpose, the THP-1wt cells were cultured in RPMI/F12 medium with 10% fetal bovine serum in the presence of 1 μM АTRA (Sigma, United States) for 96 h. Macrophage-like THP-1PMA cells were obtained by treatment with phorbol-12-myristate-13-acetate (PMA). For this purpose, the THP-1wt cells were cultured in DMEM/F12 medium with 10% fetal bovine serum in the presence of 200 nM PMA (Sigma, United States) for 96 h. Monocytes were obtained from the mononuclear fraction of peripheral human blood using a MojoSort Human Pan Monocyte Isolation Kit (BioLegend, United States) in accordance with the manufacturer’s recommendations. Macrophages were obtained from human peripheral blood monocytes. To obtain macrophages, monocytes were seeded on culture plastic and incubated for 7 days in DMEM/F12 medium with 2% fetal bovine serum.

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