StemSep™ Human CD34 Positive Selection Cocktail

Protocol tips

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	CD34+ cells were kept for 2-3 days in StemAlpha A medium (StemAlpha) in the presence of Flt3-ligand (50ng/ml), thrombopoietin (50ng/ml) and stem cell factor (50ng/ml).

Protocol tips
CD34+ cells were kept for 2-3 days in StemAlpha A medium (StemAlpha) in the presence of Flt3-ligand (50ng/ml), thrombopoietin (50ng/ml) and stem cell factor (50ng/ml).

Publication protocol

Cytapheresis samples from four CML patients were obtained at diagnosis according to the Etablissement Français du Sang recommendations. Mononuclear cells were obtained by Ficoll density gradient centrifugation. They were labeled with the StemSep™ Human CD34 Positive Selection Cocktail (Stemcell Technologies) and isolated on MS column (Miltenyi Biotec). CD34+ cells were kept for 2-3 days in StemAlpha A medium (StemAlpha) in the presence of Flt3-ligand (50ng/ml), thrombopoietin (50ng/ml) and stem cell factor (50ng/ml). Cytokines were not included during the course of imatinib treatments.

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Manufacturer protocol

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