FITC Annexin V Apoptosis Detection Kit I

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1 × 10^6 cells/mL were used for the assay.	- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Upstream tips
- 1 × 10^6 cells/mL were used for the assay.
Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Publication protocol

The Annexin V-FITC Apoptosis Detection Kit (BD Biosciences, San Jose, CA, USA) was used. In brief, the harvested cells were re-suspended in 1x binding buffer at a density of 1 × 106 cells/mL and cells of each 100 μl aliquot were stained with Annexin V-PI labeling solution (containing 5 μl Annexin V-FITC and 5 μl propidium iodide) at room temperature in the dark for 15 min. Finally, binding buffer (400 μl) was added and the cells were analyzed by flow cytometer.

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Manufacturer protocol

Download the product protocol from BD Biosciences for FITC Annexin V Apoptosis Detection Kit I below.

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