MACSprep™ HLA B/T Cell Isolation Kit, human

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	B cells and Tfhs were isolated from HBD whole blood using the MACSprep HLA B/T Cell Isolation Kit (Miltenyi Biotec SAS).

Protocol tips
B cells and Tfhs were isolated from HBD whole blood using the MACSprep HLA B/T Cell Isolation Kit (Miltenyi Biotec SAS).

Publication protocol

B cells and Tfhs were isolated from HBD whole blood using the MACSprep HLA B/T Cell Isolation Kit (Miltenyi Biotec SAS). Sorting conditions were detailed in Supplemental Material. Memory B cells (1×104) were cocultured with blood Tfhs at a 1:1 ratio with endotoxin-reduced staphylococcal enterotoxin B (100 ng/ml; Sigma-Aldrich). Belatacept was added to the cell cultures at different concentrations (10 and 40 μg/ml).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell Isolation HLA B/T using MACSprep™ HLA B/T Cell Isolation Kit, human from Miltenyibiotec.

Manufacturer protocol

Download the product protocol from Miltenyibiotec for MACSprep™ HLA B/T Cell Isolation Kit, human below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell Isolation HLA B/T using MACSprep™ HLA B/T Cell Isolation Kit, human from Miltenyibiotec. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.