MACSprep™ HLA T Cell Isolation Kit, human

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Mouse primary T cells were isolated from lymph nodes, and human T cells were isolated from the blood of healthy volunteers following university policies (MACSprep HLA T cell isolation kit, human).		They were cultured in RPMI medium (containing 2 mM glutamine, 1 mM pyruvate, nonessential amino acids, and 50 μM 2-mercaptoethanol) supplemented with 10% FBS and 1% penicillin-streptomycin.

Upstream tips
Mouse primary T cells were isolated from lymph nodes, and human T cells were isolated from the blood of healthy volunteers following university policies (MACSprep HLA T cell isolation kit, human).
Downstream tips
They were cultured in RPMI medium (containing 2 mM glutamine, 1 mM pyruvate, nonessential amino acids, and 50 μM 2-mercaptoethanol) supplemented with 10% FBS and 1% penicillin-streptomycin.

Publication protocol

"Activation of murine and human T cells.
EL4 and Jurkat cells were cultured in RPMI medium (BioWest) supplemented with 10% fetal bovine serum (FBS; Biochrom) and 1% penicillin-streptomycin (BioWest) and were activated with PMA (20 ng/ml). Mouse primary T cells were isolated from lymph nodes, and human T cells were isolated from the blood of healthy volunteers following university policies (MACSprep HLA T cell isolation kit, human). They were cultured in RPMI medium (containing 2 mM glutamine, 1 mM pyruvate, nonessential amino acids, and 50 μM 2-mercaptoethanol) supplemented with 10% FBS and 1% penicillin-streptomycin. Cells at a density of ∼2 × 105 cells/ml were activated with coated anti-CD3 antibody (1 μg/ml) and anti-CD28 antibody (1 μg/ml)."

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