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Human CD3+ T cells were isolated from fresh whole donor blood in K2EDTA tubes using RosetteSep HLA T Cell Enrichment Cocktail according to instructions of the manufacturer (Stemcell). |
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Protocol tips |
Human CD3+ T cells were isolated from fresh whole donor blood in K2EDTA tubes using RosetteSep HLA T Cell Enrichment Cocktail according to instructions of the manufacturer (Stemcell). |
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Publication protocol
"T cell isolation
Human CD3+ T cells were isolated from fresh whole donor blood in K2EDTA tubes using RosetteSep HLA T Cell Enrichment Cocktail according to instructions of the manufacturer (Stemcell). Isolated CD3+ T cells were enumerated in a haemocytometer with 0.4% trypan blue (Sigma). CD3+ T cells were then used in the ICS and proliferation co-cultures."
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