NucleoSpin® Soil

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Buffers A3 and AW contain guanidine hydrochloride which can form highly reactive compounds when combined with bleach (sodium hypochlorite). so DO NOT add bleach or acidic solutions directly to the sample preparation waste.

Upstream tips
Buffers A3 and AW contain guanidine hydrochloride which can form highly reactive compounds when combined with bleach (sodium hypochlorite). so DO NOT add bleach or acidic solutions directly to the sample preparation waste.

Publication protocol

Kit selection and DNA extraction
DNA extraction was performed using four commercial kits following the manufacturer's instructions. The kits, which used silica spin filter technology, were chosen for their easy and fast extraction methods. The following kits were evaluated: PowerSoil® DNA isolation and PowerFecal® kits (Mo Bio Laboratories Inc., Carlsbad, CA), QIAamp® DNA stool mini kit (Qiagen Inc., Valencia, CA), and NucleoSpin® Soil (Macherey-Nagel, Düren, Germany). For NucleoSpin® Soil, sample lysis was performed with the optional enhancer SX solution, as recommended by the manufacturer. In the rest of this article, the four kits are referred as PS, PF, QI, and NS, respectively.

To enable direct comparison of the kits, the same amount of starting material was used. DNA was extracted either from 250 mg of raw manure or from 250 mg of pellet after centrifugation of 90 mL of lagoon effluent at 16,000g for 30 min.

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Reviews

Discussion

Papers

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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® Soil below.

Download manufacturer protocol

Videos

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