Naive Pan T Cell Isolation Kit, human

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	T cells were purified by magnetic bead selection using the Miltenyi pan-T cell isolation kit (Miltenyi Biotec, Auburn, CA). CLA− and CLA+ subsets were isolated by subsequent incubation of T cells in biotin anti-human CLA (BD Pharmingen) followed by anti-biotin microbeads and magnetic separation (Miltenyi).	Positively selected cells were collected as the CLA+-enriched fraction and negatively selected cells were collected as the CLA− subset.

Protocol tips
T cells were purified by magnetic bead selection using the Miltenyi pan-T cell isolation kit (Miltenyi Biotec, Auburn, CA). CLA− and CLA+ subsets were isolated by subsequent incubation of T cells in biotin anti-human CLA (BD Pharmingen) followed by anti-biotin microbeads and magnetic separation (Miltenyi).
Downstream tips
Positively selected cells were collected as the CLA+-enriched fraction and negatively selected cells were collected as the CLA− subset.

Publication protocol

Peripheral blood mononuclear cells were obtained as discarded pheresis material from red blood cell donors. T cells were purified by magnetic bead selection using the Miltenyi pan-T cell isolation kit (Miltenyi Biotec, Auburn, CA). CLA− and CLA+ subsets were isolated by subsequent incubation of T cells in biotin anti-human CLA (BD Pharmingen) followed by anti-biotin microbeads and magnetic separation (Miltenyi). Positively selected cells were collected as the CLA+-enriched fraction and negatively selected cells were collected as the CLA− subset. CLA− memory and naïve cells were then separated by use of anti-CD45RO microbeads and magnetic separation (Miltenyi).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell Isolation Naive Pan T cell using Naive Pan T Cell Isolation Kit, human from Miltenyibiotec.

Manufacturer protocol

Download the product protocol from Miltenyibiotec for Naive Pan T Cell Isolation Kit, human below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell Isolation Naive Pan T cell using Naive Pan T Cell Isolation Kit, human from Miltenyibiotec. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.