EasySep™ Human Naïve Pan T Cell Isolation Kit

Protocol tips

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	T cells were isolated (at >95% purity as determined by CD3 expression) via magnetic-activated cell sorting (MACS) using the Naïve Pan T Cell Isolation Kit per the manufacturer’s instructions (Stemcell Technologies) and activated with a non-specific activation stimuli method

Protocol tips
T cells were isolated (at >95% purity as determined by CD3 expression) via magnetic-activated cell sorting (MACS) using the Naïve Pan T Cell Isolation Kit per the manufacturer’s instructions (Stemcell Technologies) and activated with a non-specific activation stimuli method

Publication protocol

"Human peripheral blood mononuclear cells (PBMCs) were obtained from freshly obtained buffy
coat fractions (Stanford Blood Center) using Ficoll-Paque Plus following the manufacturer’s
instructions (GE Healthcare). T cells were isolated (at >95% purity as determined by CD3
expression) via magnetic-activated cell sorting (MACS) using the Naïve Pan T Cell Isolation Kit
per the manufacturer’s instructions (Stemcell Technologies) and activated with a non-specific activation stimuli method; Phorbol 12-myristate 13-acetate (PMA) (Abcam) and Ionomycin
(Abcam) were added at a concentration of 10 ng/ml and 100ng/ml respectively. Cells were used
for FACs analysis, 48 hours post initiation of activation. "

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Manufacturer protocol

Download the product protocol from STEMCELL technologies for EasySep™ Human Naïve Pan T Cell Isolation Kit below.

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