QIAamp DNA Stool Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- When using frozen stool samples, take care that the samples do not thaw until Buffer ASL is added in step 2 to lyse the sample; otherwise the DNA in the sample may degrade.

Upstream tips
- When using frozen stool samples, take care that the samples do not thaw until Buffer ASL is added in step 2 to lyse the sample; otherwise the DNA in the sample may degrade.

Publication protocol

Kit selection and DNA extraction
DNA extraction was performed using four commercial kits following the manufacturer's instructions. The kits, which used silica spin filter technology, were chosen for their easy and fast extraction methods. The following kits were evaluated: PowerSoil® DNA isolation and PowerFecal® kits (Mo Bio Laboratories Inc., Carlsbad, CA), QIAamp® DNA stool mini kit (Qiagen Inc., Valencia, CA), and NucleoSpin® Soil (Macherey-Nagel, Düren, Germany). For NucleoSpin® Soil, sample lysis was performed with the optional enhancer SX solution, as recommended by the manufacturer. In the rest of this article, the four kits are referred as PS, PF, QI, and NS, respectively.

To enable direct comparison of the kits, the same amount of starting material was used. DNA was extracted either from 250 mg of raw manure or from 250 mg of pellet after centrifugation of 90 mL of lagoon effluent at 16,000g for 30 min.

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus using QIAamp DNA Stool Mini Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Stool Mini Kit below.

Download manufacturer protocol

Videos

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