Publication protocol
"Isolation of B-cells from mononuclear cells using the Miltenyi cell isolation kits
Miltenyi isolation kits are based on a simple process, in which biotin conjugated antibody-labelled mononuclear cells are separated from unlabeled cells via a column in the presence of a magnetic field. During negative selection, the cell type of interest (in this case B-cells) remains unlabeled/“untouched” and in its native state, while the remaining unwanted mononuclear cells (in this case T-cells, NK cells and monocytes) are targeted via biotin labelled-antibodies specific for cell surface receptor(s) of those cell types. During positive selection, the cell type of interest (in this case B-cells) are specifically targeted, labelled and actively magnetically removed from the sample by retention in the column in the presence of a magnetic field. These cells are then retrieved by plunging the column in the absence of a magnetic field.
The efficiency of various Miltenyi isolation kits were tested. Specifically, 3 negative B-cell isolation kits, 1 positive B-cell isolation kit and 1 T-cell isolation kit were examined (see list below). All kits were operated according to the manufacturer’s instructions. For a more detailed description regarding this process refer to dx.doi.org/10.17504/protocols.io.yfzftp6 [PROTOCOL DOI] List of Miltenyi isolation kits examined
Isolation of B-cells from mononuclear cells by negative selection using the Miltenyi B-cell Isolation kit II (For detailed description of kit components see Datafile A in S1 File)
Isolation of B-cells from mononuclear cells by negative selection using the Miltenyi Naïve B-cell Isolation kit (For detailed description of kit components see Datafile D in S1 File)
Isolation of B-cells from mononuclear cells by negative selection using the Miltenyi CD43 Microbeads kit (For detailed description of kit components see Datafile E in S1 File)
Isolation of B-cells from mononuclear cells by positive selection using the Miltenyi CD19 positive Isolation kit (For detailed description of kit components see Datafile F in S1 File)"
Full paper
Login or
join for free to view the full paper.
Videos
Check out videos that might be relevant for performing Cell Isolation B cell using B Cell Isolation Kit II, human from Miltenyibiotec. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.