MagCellect Human B Cell Isolation Kit

Cell Isolation B cell

5/5 - 1 Review - 100% recommends this

Experiment
Cell Isolation B cell
Product
MagCellect Human B Cell Isolation Kit from R&D Systems
Manufacturer
R&D Systems

Protocol tips

Protocol tips
purified B cells (>95%) were isolated using MagCellect Human B Cell Isolation Kit (R&D systems, USA). B cell purity was assessed by flow cytometry using APC labeled CD19 antibody (Biolegend, USA).

Publication protocol

Blood samples were collected in heparinized tubes, and were diluted 1:1 with serum-free RPMI 1640 (Gibco, USA) medium. Peripheral blood mononuclear cells (PBMCs) were separated by Ficoll (GE Healthcare, USA) density gradient centrifugation. For RNA isolation and Western blotting, purified B cells (>95%) were isolated using MagCellect Human B Cell Isolation Kit (R&D systems, USA). B cell purity was assessed by flow cytometry using APC labeled CD19 antibody (Biolegend, USA). For transfection and cell culture, PBMCs were obtained from healthy volunteers.

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Protocol
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Product rating
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Yes

What worked well?
  • the kit worked fine for me
What could be improved?
  • NA

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Manufacturer protocol

Download the product protocol from R&D Systems for MagCellect Human B Cell Isolation Kit below.

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