MojoSort™ Human B Cell (CD43-) Isolation Kit

Protocol tips

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	Human CD43− resting B cells were isolated from PBMCs using MojoSort Human B Cell (CD43−) Isolation Kit (BioLegend), according to the manufacturer's protocols.	Isolated resting B cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 10 mM HEPES, 100 μg/ml streptomycin, 100 U/ml penicillin, and 1 mM 2-mercaptoethanol.

Protocol tips
Human CD43− resting B cells were isolated from PBMCs using MojoSort Human B Cell (CD43−) Isolation Kit (BioLegend), according to the manufacturer's protocols.
Downstream tips
Isolated resting B cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 10 mM HEPES, 100 μg/ml streptomycin, 100 U/ml penicillin, and 1 mM 2-mercaptoethanol.

Publication protocol

Human PBMCs were separated by density gradient centrifugation using Lympholyte-H (Cedarlane, Burlington, Canada). Human CD43− resting B cells were isolated from PBMCs using MojoSort Human B Cell (CD43−) Isolation Kit (BioLegend), according to the manufacturer's protocols. Isolated resting B cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 10 mM HEPES, 100 μg/ml streptomycin, 100 U/ml penicillin, and 1 mM 2-mercaptoethanol. For some experiments, resting B cells were stimulated with 1 μg/ml anti-human IgM Ab (BioLegend), 1 μg/ml recombinant human CD40L (BioLegend), 100 μg/ml poly(I:C), and/or 50 μg/ml imiquimod (R837; InvivoGen, San Diego, CA, USA). After incubation for 24 h, the cells were immediately used for flow cytometric analyses, and the supernatants were stocked at – 80°C until use.

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Manufacturer protocol

Download the product protocol from BioLegend for MojoSort™ Human B Cell (CD43-) Isolation Kit below.

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