EasySep™ Direct Human B Cell Isolation Kit

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	Primary B-cells were purified from peripheral blood using EasySep™ Direct Human B Cell Isolation Kit from Stemcells Technologies Cat #19674 and directly used for RNA purification.

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Protocol tips
Primary B-cells were purified from peripheral blood using EasySep™ Direct Human B Cell Isolation Kit from Stemcells Technologies Cat #19674 and directly used for RNA purification.

Publication protocol

COX cells (13) were obtained from the International Histocompatibility Working Group, Seattle, WA, USA [(IHW09022) http://www.ihwg.org/hla/index.html]. PGF cells (13) were obtained from the Coriell Biorepository (Cat #GM03107). Cells were culured in RPMI-1640 medium with 15% FBS (Sigma Cat #F2442-500ML). HEK 293T cells (a gift from Xianxin Hua in the Department of Cancer Biology at the University of Pennsylvania, Perelman School of Medicine) were cultured in DMEM (Cat #10-013-CV) media with 10% FBS. Primary B-cells were purified from peripheral blood using EasySep™ Direct Human B Cell Isolation Kit from Stemcells Technologies Cat #19674 and directly used for RNA purification. Selective IgA-deficiency patient cell lines (ID 000018, ID000036, ID000038, and ID000057) and cell lines from unaffected, related family members (ID000037 and ID000058) were originally collected and characterized as part of an initiative by the US Immunodeficiency Network and purchased from the Coriell Biorepository.

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