AmpFLSTR™ Identifiler™ PCR Amplification Kit

Cell line authentication Colon cancer cell line LoVo

Experiment
Cell line authentication Colon cancer cell line LoVo
Product
AmpFLSTR™ Identifiler™ PCR Amplification Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Isolate DNA using phenol/chloroform, or magnetic beads (the Maxwell 16 DNA Purification kits, Promega), and MagAttract DNA Mini M48 kit, Qiagen)
Protocol tips
Match STR profiles with the ATCC's and German Collection of Microorganisms and Cell cultures' (Braunschweig, Germany) online databases

Publication protocol

Colon cancer cell lines: Twenty-seven colon cancer cell lines were initially included in the present study. HCT-116, HCT-15, LoVo, RKO, SW1116, SW48, SW620, SW948, NCI-H508 and WiDr were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). ALA, CaCo-2, CO-115, COLO 320, DLD-1, EB, FRI, HT-29, IS1, IS2, IS3, LS1034, LS-174T, TC7, TC71, SW480 and V9P were kindly provided by collaborators. Cell lines were cultured in medium with added fetal bovine serum, glutamine, penicillin and streptomycin, and were maintained in humidified 37 °C 5% CO2 incubators as described in Supplementary Table 2. Before collection, cultures were tested for mycoplasma infection using Myco Alert (Lonza, Walkersville, MD, USA) according to the manufacturer's protocol. DNA was isolated using either a standard phenol/chloroform procedure, or a magnetic beads approach (the Maxwell 16 DNA Purification kits, Promega Corporation, Madison, WI, USA, and MagAttract DNA Mini M48 kit, Qiagen Inc., Valencia, CA, USA). DNA was STR profiled using the AmpFLSTR Identifiler PCR Amplification Kit (Life Technologies, Carlsbad, CA, USA). Resulting cancer cell line STR profiles were cross-compared and, where available, matched with the ATCC's and German Collection of Microorganisms and Cell cultures' (Braunschweig, Germany) online databases. Hierarchical clustering of STR data was performed using Euclidian distances and average linkage clustering in Partek Genomics Suite 6.6 (Partek Inc., St Louis, MO, USA; Figure 2). ALA, CO-115, EB, FRI, IS1, IS2, IS3, TC7, TC71 and V9P are non-commercial cell lines and their STR profiles will be provided upon request. Three of the 27 cancer cell lines were found to be misclassified. ALA and IS2 had identical profiles to SW480/SW620 and LS1034, respectively. TC7 had a STR profile incompatible with its origin as a Caco-2 subclone.41 Consequently, ALA, IS2 and TC7 were excluded from further analysis.

Full paper   Login or join for free to view the full paper.

Reviews

AmpFLSTR™ Identifiler™ PCR Amplification Kit from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell line authentication Colon cancer cell line LoVo using AmpFLSTR™ Identifiler™ PCR Amplification Kit from Thermo Fisher Scientific.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for AmpFLSTR™ Identifiler™ PCR Amplification Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell line authentication Colon cancer cell line LoVo using AmpFLSTR™ Identifiler™ PCR Amplification Kit from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms