AmpFLSTR™ Identifiler™ PCR Amplification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

Cell culture: Cells were maintained in a humidified incubator at 37C with 5% CO2. See Table S1 for a list of cell lines, culture conditions and contributing labs and repositories. Some cell lines were derived in-house (labeled with “VOA#”) through continuous in vitro culture of primary patient material obtained through the OVCARE Tumor bank. All patients with tissue deposited in the OVCARE tumor bank provided written consent for experimental studies including sequencing, IHC characterization, and derivation of long-term cell lines from tissue samples. The OVCARE tumor bank study was approved under University of British Columbia and British Columbia Cancer Agency Research Ethics Board H05-60119 protocol.
All cell lines were subjected to identity testing using STR genotyping (AmpFlSTR Identifiler, Applied Biosystems) at the College of American Pathologist's (CAP) accredited Centre For Translational and Applied Genomics (CTAG) as per manufacturer directives. Only lines with profiles matching public repository records, reported STR [32], and/or original patient tumors (in the case of in-house derived cell lines) were retained for further study.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell line authentication Ovarian adenocarcinoma cell line A2780 using AmpFLSTR™ Identifiler™ PCR Amplification Kit from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for AmpFLSTR™ Identifiler™ PCR Amplification Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell line authentication Ovarian adenocarcinoma cell line A2780 using AmpFLSTR™ Identifiler™ PCR Amplification Kit from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.