PowerPlex® 16 System

Protocol tips

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	Follow the manufacturer’s instructions Compare results with official entries of different cell lines in the STR profile database maintained by DSMZ

Protocol tips
Follow the manufacturer’s instructions Compare results with official entries of different cell lines in the STR profile database maintained by DSMZ

Publication protocol

STR profiling: To identify the different cell lines and stocks the PowerPlex 16 Kit (Promega, Madison, WI) was used for STR profiling. The STR profiling was performed according to the manufacturer's instructions using the protocols for the GeneAmp® PCR System 2400 Thermal Cycler (Perkin Elmer/Applied Biosystems, Waltham, MA) and the ABI PRISM® 310 Genetic Analyzer (Perkin Elmer/Applied Biosystems).STR profiles were analyzed with the software package Genemapper 4.1 (Applied Biosystems, Carlsbad, CA). The STR profile of UROtsa cell had been determined before but was never published in detail [5]. A. Fabarius kindly provided us with this previous analysis of UROtsa-4 cells that had been performed at the DSMZ in 2009. Results were compared with official entries of different cell lines in the STR profile database maintained by DSMZ (German Collection of Microorganisms and Cell Cultures, www.dsmz.de).

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Manufacturer protocol

Download the product protocol from Promega for PowerPlex® 16 System below.

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