MethylCap kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Always thaw the MethylCap protein on ice. Preferably aliquots it to avoid multiple freeze-thaw cycles.

Protocol tips
Always thaw the MethylCap protein on ice. Preferably aliquots it to avoid multiple freeze-thaw cycles.

Publication protocol

Kit selection and methylated DNA capturing:
All MBD-based capturing kits commercially available at the time of study initiation were included in this study. Tested kits, with indication of used MBDs (although typically recombinant forms), were: MethylMagnet™ mCpG DNA isolation kit (MBD2b) (Ribomed MM101-K, Carlsbad, California, USA), MethylCollector™ (MBD2b) and MethylCollector™ Ultra (MBD2b and MBD3L1) (Active Motif 55005, Carlsbad, California, USA), MethylCap™ kit (MBD from MeCP2) (Diagenode AF-100-0048, Liège, Belgium), MethylMiner™ Methylated DNA Enrichment Kit (MBD2) (Invitrogen ME10025, Merelbeke, Belgium). For the remainder of the manuscript, these kits are referred to as respectively MethylMagnet, MethylCollector, MethylCollector Ultra, MethylCap and MethylMiner kits. Frequency of use, as estimated by Google Scholar citations () at the beginning of January 2013, were roughly 50 times for the MethylCollector kits, 25 times for MethylCap and 50 times for MethylMiner, whereas only 3 references to the MethylMagnet kit could be found.

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Whole genome profiling - DU145, PC3 human prostate cancer using MethylCap kit from Diagenode.

Manufacturer protocol

Download the product protocol from Diagenode for MethylCap kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Whole genome profiling - DU145, PC3 human prostate cancer using MethylCap kit from Diagenode. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.