AmpFLSTR™ Identifiler™ PCR Amplification Kit

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	Compare profiles to consolidated (ATCC, DSMZ, JCRB and RIKEN) databases

Protocol tips
Compare profiles to consolidated (ATCC, DSMZ, JCRB and RIKEN) databases

Publication protocol

DNA isolation and STR profiling: Cell lines were grown in appropriate specific standard media. Genomic DNA was isolated from 0.5–5 × 106 cells using a Zymo Research ZR genomic DNA II kit and quantified by gel electrophoresis and ethidium bromide staining by comparison to a DNA mass ladder. Multiplex PCR amplified products were generated using 1–2 ng of genomic DNA with an Applied Biosystems Identifiler kit and ABI 3730 capillary sequencer as described [2, 18]. STR loci were analyzed with Gene Mapper 4.0. Profiles were compared to published reports [22, 25], consolidated (ATCC, DSMZ, JCRB and RIKEN) databases, and an in-house database, using a custom search algorithm designed to facilitate comparison of cell lines with related profiles and identify individual cell lines in a mixture (C. Korch and J. West, Vanderbilt University, unpublished). STR profiles of the ovarian and endometrial cancer cells analyzed in this study are available online at http://DNAsequencingcore.UCDenver.edu.

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