PowerPlex® 16 HS System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Isolate DNA from cell lines using a standard DNA isolation kit	Follow the manufacturer’s protocol Compare the STR profiles to STR-fingerprints from the German Collection of Microorganisms and Cell Cultures (DSMZ) database

Upstream tips
Isolate DNA from cell lines using a standard DNA isolation kit
Protocol tips
Follow the manufacturer’s protocol Compare the STR profiles to STR-fingerprints from the German Collection of Microorganisms and Cell Cultures (DSMZ) database

Publication protocol

STR Fingerprinting: DNA was isolated from cell lines using a standard DNA isolation kit and from paraffin embedded tissue sections using the Ex-Wax DNA extraction kit (Millipore). The DNA fingerprinting was performed by STR profiling. DNA amplifications were made using the PowerPlex 16 HS kit (Promega) according to the manufacturer's recommendations. The primers of the kit amplify 15 tetranucleotide repeat loci plus the amelogenin (AMEL) sex-determining marker. The combination of this set of markers is in accordance with worldwide database recommendations of identity testing.5 A genetic analyzer ABI 3100 (Applied Biosystems) was used to separate and identify the alleles using standard procedures. The results were confirmed in an independent experiment. For comparisons, STR-fingerprints from cell lines were downloaded from the German Collection of Microorganisms and Cell Cultures (DSMZ) database (http://www.dsmz.de/fp/cgi-bin/str.html), which comprises 9 marker profiles (8 STR markers plus the AMEL marker) of 2289 cell lines from DSMZ, American Type Cell Culture (ATCC), Japanese Collection of Research Bioresources (JCRB), and RIKEN. In addition, we obtained the 16 marker (15 STR + AMEL) profiles of the NCI-60 cell line panel that has been published recently.5 These profiles were established with the same standard marker set used in this study.

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Manufacturer protocol

Download the product protocol from Promega for PowerPlex® 16 HS System below.

Download manufacturer protocol

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