QuantiTect Probe RT-PCR Kit (1000)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Follow manufacturer's instructions

Protocol tips
Follow manufacturer's instructions

Publication protocol

TaqMan RT-rtPCR assays: RT-rtPCR protocol: RT-rtPCR was performed using a total volume of 25 µL on 2 µL of each RNA dilution and 23 µL of the master mix components from each tested kit. The concentrations of the forward and reverse primers and the Lys-Gt1 probe were optimized for each tested kit.21
All commercial kits were optimized with regard to the reverse transcription duration according to the manufacturer’s instructions (Table 1). The same PCR thermal cycling conditions (Table 1) were applied for the 6 kits tested (i.e., 45 cycles of 30 s at 94°C, 30 s at 55°C, and 30 s at 72°C). Real-time PCRs were performed on 2 real-time thermocyclers: Rotor-Gene Q MDx (RG, Qiagen) and Mx3005P (Agilent Technologies, Les Ulis, France). PCR runs were performed in independent assays on multiple days by one trained laboratory technician. Preparation of PCR master mixes was performed by manual pipetting.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing PCR Quantitative real-time PCR - Viral using QuantiTect Probe RT-PCR Kit (1000) from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for QuantiTect Probe RT-PCR Kit (1000) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing PCR Quantitative real-time PCR - Viral using QuantiTect Probe RT-PCR Kit (1000) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.