QuantiTect Virus Kit (1000)

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Protocol tips
Follow manufacturer's instructions

Publication protocol

TaqMan RT-rtPCR assays: RT-rtPCR protocol: RT-rtPCR was performed using a total volume of 25 µL on 2 µL of each RNA dilution and 23 µL of the master mix components from each tested kit. The concentrations of the forward and reverse primers and the Lys-Gt1 probe were optimized for each tested kit.21
All commercial kits were optimized with regard to the reverse transcription duration according to the manufacturer’s instructions (Table 1). The same PCR thermal cycling conditions (Table 1) were applied for the 6 kits tested (i.e., 45 cycles of 30 s at 94°C, 30 s at 55°C, and 30 s at 72°C). Real-time PCRs were performed on 2 real-time thermocyclers: Rotor-Gene Q MDx (RG, Qiagen) and Mx3005P (Agilent Technologies, Les Ulis, France). PCR runs were performed in independent assays on multiple days by one trained laboratory technician. Preparation of PCR master mixes was performed by manual pipetting.

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Manufacturer protocol

Download the product protocol from Qiagen for QuantiTect Virus Kit (1000) below.

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