QuantiFast Pathogen PCR +IC Kit (400)

Protocol tips

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	Test different primer/probe concentrations and extension temperatures in order to achieve maximum replication efficiency.

Protocol tips
Test different primer/probe concentrations and extension temperatures in order to achieve maximum replication efficiency.

Publication protocol

The Real-Time PCRs were carried out using the QuantiFast® Pathogen PCR + IC Kit (QIAGEN, Hilden, Germany). This kit includes an Internal Amplification Control (IAC), as well as specific reagents and primers/probes required for its amplification. It employs MAX™NHS Ester as a reporter dye. Different primer/probe concentrations and extension temperatures were tested in order to achieve maximum replication efficiency.

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Manufacturer protocol

Download the product protocol from Qiagen for QuantiFast Pathogen PCR +IC Kit (400) below.

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