QuantiNova SYBR Green RT-PCR Kit (2500)

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Publication protocol

Real-time RT-PCR assay for SARS-CoV-2 RNA detection was performed using QuantiNova SYBR Green RT-PCR Kit (QIAGEN, Hilden, Germany) and a LightCycler 480 II real-time PCR System (Roche, Basel, Switzerland) [4,20]. Each 20 μL reaction mixture contained 10 μL of 2× QuantiNova SYBR Green RT-PCR Master Mix, 0.2 μL of QN SYBR Green RT-Mix, 1 μL of each 10 μM forward and reverse primer, 2.8 μL of RNase-free water and 5 μL of TNA as the template. The thermal cycling condition was 10 min at 50 °C and 2 min at 95 °C, followed by 45 cycles of 5 s at 95 °C and 10 s at 60 °C, and then subjected to melting curve analysis (95 °C for 5 s, 65 °C for 1 min, followed by a gradual increase in temperature to 97 °C with continuous recording of fluorescence).

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