QuantiNova SYBR Green RT-PCR Kit (2500)

PCR Quantitative real-time PCR - Viral

Experiment
PCR Quantitative real-time PCR - Viral
Product
QuantiNova SYBR Green RT-PCR Kit (2500) from Qiagen
Manufacturer
Qiagen

Protocol tips

Publication protocol

Real-time RT-PCR assay for SARS-CoV-2 RNA detection was performed using QuantiNova SYBR Green RT-PCR Kit (QIAGEN, Hilden, Germany) and a LightCycler 480 II real-time PCR System (Roche, Basel, Switzerland) [4,20]. Each 20 μL reaction mixture contained 10 μL of 2× QuantiNova SYBR Green RT-PCR Master Mix, 0.2 μL of QN SYBR Green RT-Mix, 1 μL of each 10 μM forward and reverse primer, 2.8 μL of RNase-free water and 5 μL of TNA as the template. The thermal cycling condition was 10 min at 50 °C and 2 min at 95 °C, followed by 45 cycles of 5 s at 95 °C and 10 s at 60 °C, and then subjected to melting curve analysis (95 °C for 5 s, 65 °C for 1 min, followed by a gradual increase in temperature to 97 °C with continuous recording of fluorescence).

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Discussion

Discussion

5 years ago

Author: Germany

What is the optimal concentration for primers in qPCR?

What is the optimal concentration for primers in qPCR? My total volume is 20μl per reaction.

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for QuantiNova SYBR Green RT-PCR Kit (2500) below.

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