QuantiTect Whole Transcriptome Kit (100)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	To receive high cDNA yield, perform a high-yield reaction consisting of an 8 h amplification step

Protocol tips
To receive high cDNA yield, perform a high-yield reaction consisting of an 8 h amplification step

Publication protocol

Whole Transcriptome Amplification: For unlimited gene expression analysis, the QuantiTect Whole Transcriptome Kit (Qiagen) was used according to manufacturer’s instructions. This method synthesizes cDNA from total RNA by Whole Transcriptome Amplification and comprises three steps: reverse transcription, cDNA ligation and amplification. About 10 up to 100 ng of total RNA were added to RT mix. Furthermore, the high-yield reaction consisting of an 8 h amplification step was performed to receive high cDNA yield. Corresponding to the QuantiTect Whole Transcriptome Handbook, the amplified cDNA was diluted 1:250 with water, and directly used for RT- and qPCR analysis.

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Manufacturer protocol

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