REPLI-g Cell WGA & WTA Kit (48)

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	Follow manufacturer’s instructions

Protocol tips
Follow manufacturer’s instructions

Publication protocol

Amplification.Nucleic acid amplification was performed using the REPLI-g WGA & WTA kit (Qiagen) per the manufacturer's package insert, with the omission of the cell lysis step, which had been performed previously using the MagMAX kit (see above). Subsamples A, F, I, and L underwent whole-transcriptome amplification prior to RNASeq (referred herein as the cDNA method). Subsamples B, D, E, G, J, and M underwent whole-genome amplification prior to DNASeq (referred herein as the DNAamp method). Subsamples C, H, and K did not undergo any amplification prior to library preparation for DNASeq (referred herein as the DNA method).

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