REPLI-g FFPE Kit (100)

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Publication protocol

Isolation and WGA of Genomic DNA from the Cell Line, WBCs, and FFPE Tissues: Genomic DNA from WBCs was isolated using the QIAamp DNA Blood Mini kit (Qiagen, Germantown, MD, USA). For bulk cancer cells, an AllPrep DNA/RNA/Protein kit (Qiagen, Germantown, MD, USA) was used as per the manufacturer’s instructions. The DNA extractions from FFPE samples were made from a 10 µm thick section using the GeneRead DNA FFPE kit (Qiagen, Germantown, MD, USA), as per the recommended instructions. The WGA of spike-in and clinical samples was conducted using the Repli-g Single cell kit (Qiagen, Germantown, MD, USA), according to the manufacturer’s protocol. For FFPE samples, the Repli-g FFPE kit (Qiagen Germantown, MD, USA) was used for WGA. All the DNA samples were quantified using a NanoDrop-2000 (ThermoFisher Scientific, Carlsbad, CA, USA) and Qubit 2.0 Fluorometer (ThermoFisher Scientific Inc., Waltham, MA, USA). WGA quality control was performed by Ampli1 QC kit (Menarini Silicon Biosystems, Huntingdon Valley, PA, USA) to check DNA integrity by conducting multiplex PCR of 4 targets at chromosome 12p, 5p, 17p, and 6p.

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