REPLI-g Single Cell Kit (96)

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	Follow manufacturer’s instructions

Protocol tips
Follow manufacturer’s instructions

Publication protocol

Ampli-1 WGA, REPLI-g WGA, DOPlify WGA: Cell lysis and amplification was performed, using the Ampli-1 WGA kit (Silicon Biosystems, Castel Maggiore, Italy), the REPLI-g single cell kit (Qiagen Hilden, Germany) or the DOPlify WGA kit (Reproductive Health Science, Thebarton, Australia) as described in the respective manufacturer’s instructions. All samples were purified following the manufacturer’s protocol of the Genomic DNA Clean & Concentrator kit (version 1.0.0, Zymo Research, Irvine, USA) with 5X binding buffer. Concentration was measured using the Qubit dsDNA High Sensitivity Assay kit (Life technologies, Carlsbad, USA).

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Manufacturer protocol

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